In this paper the influence of factors affecting gynogenic response of red beet ovules is discussed.The ovule response frequencies Hair Busters were the highest in the following conditions: N6 (Chu 1975) mineral salts, 0.5 mg/l IAA, 0.
2 mg/l BA, 27 or 32oC.The influence of genotype of donor plants was confirmed and it was found that the ovules excised from cultivar plants have Girls Tops a greater gynogenic ability than the ovules of hybrids or inbred lines.